| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Effect of Silk Contents on the Attachment and Proliferation of Retinal Pigment Epithelial Cells on Silk/PLGA Films |
| 초록 |
Biomaterials for retinal tissue engineering must demonstrate several critical features for potential utility, mechanical integrity, biocompatibility and slow biodegradation. Silk is being rediscovered and reconsidered as potentially useful biomaterials in a range of applications. We prepared natural/synthetic hybrid silk/PLGA films using 0, 10, 20, 40 and 80 wt% of silk by the solvent evaporation method. We measured MTT assay to confirm cellular of cell number attached on film at 1, 2 and 3 days. Morphology of cellular adhesion on films was confirmed by scanning electron microscope (SEM) at 1, 2 and 3 days. RT-PCR was conducted to confirm mRNA expression of RPE65 as RPEs marker. The expression of cytokeratin was determined by immunofluorescence staining. We confirmed that specific 20~40 wt% silk in silk/PLGA film was superior to adhesion and proliferation of RPEs. This research was supported by WCU (R31-20029) and SCRC (SC4110). |
| 저자 |
유현1, 이가영1, 강수지1, 유한나1, 김은영1, 주천기2, 이동원1, 강길선1
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| 소속 |
1전북대, 2가톨릭대 |
| 키워드 |
Retinal Pigment Epithelial; PLGA
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| E-Mail |
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