| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Post-probe attached hydrogel microparticle for multiplexed miRNA detection |
| 초록 |
Recently, encoded hydrogel particles have gained much attention as these particles enable high performance multiplexed miRNA assays. However, due to the low conversion of hydrogel microparticle synthesis step, current microparticles can incorporate only about ~10% of input ssDNA probes. Here, we presented a novel post-probe attached hydrogel microparticles to overcame the limitation of current particles. By discovering that the hydrogel microparticles contain the unconverted, yet reactive double bonds inside the network, we covalently anchored the ssDNA probes after the particle synthesis. With this method, probe density increased about 8.2 times compared to that of conventional particles. We demonstrated that the increased probe density leads to better assay capability. We also demonstrate a potential application of post-probe attached hydrogel microparticles by conducting multiplexed miRNA detection in human plasma samples. |
| 저자 |
노윤호1, 이현지1, 문현준1, 김선민2, 봉기완1
|
| 소속 |
1고려대, 2서울특별시 보라매병원 |
| 키워드 |
microparticles; miRNA; post-probe attachment process
|
| E-Mail |
|
