| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
In Vitro and In Vivo Gene Delivery Using Branched Poly(Ethylene imine)-graft-Carboxymethyl Chitosan Copolymer |
| 초록 |
We conjugated O-Carboxymethylation Chitosan (OCMCh) with Poly(Ethylene imine) (MW 25,000) (PEI). To enchance the low tranfection efficiency of chitosan and reduce the high cytotoxicity of PEI. The physicochemical characterisitc of OCMPEI was investigated by 1H-NMR. Particle size distribution of the OCMPEI/pDNA complexes nanoparticles, were determined 410 ~ 195 nm using by the dynamic light scattering(DLS) technique. Gel electrophoresis of the OCMPEI/pDNA complexes were confirmed that binding affinity, pDNA protection affinity and release of pDNA. As a results, OCMPEI was good biding affinity, good protection capacity of pDNA. OCMPEI 15% was best of transfection efficiency of pDNA in cancer cell line by fluorescence microscopy. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(2011-0012039). |
| 저자 |
김영민, 김준호, 김태훈, 박준규, 나재운, 장미경
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| 소속 |
순천대 |
| 키워드 |
pDNA; chitosan; PEI; GDS.
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| E-Mail |
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