| 초록 |
The Escherichia coli K-12 and B strains are among the most frequently used bacterial hosts. However, both strains have distinctive differences in genotypic and phenotypic attributes, and their behaviors can still be unpredictable at times, especially while expressing a recombinant protein. In our previous study, we identified a limited number of proteins from the two strains using two-dimensional gel electrophoresis and tandem mass spectrometry. Here, an in-depth analysis of the physiological behavior of E. coli K-12 and B strains on the proteomic level was performed using isobaric tandem mass tag (TMT)-based quantitative mass spectrometry. In this poster, distinctive differences in the proteomes between the two strains will be presented. The resulting enhancement of knowledge regarding host physiology that is summarized herein is expected to contribute to the acceleration of strain improvements and optimization for biotechnology-related processes. [This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2010-0008826 and 2015R1D1A1A01057315)] |
