| 학회 |
한국화학공학회 |
| 학술대회 |
2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 |
9권 2호, p.1940 |
| 발표분야 |
분리기술 |
| 제목 |
Refolding of fusion ferritin using gel filtration chromatography |
| 초록 |
Fusion ferritin(FH+FL), an iron-binding protein, was purified from recombinant E. coli by two-step sonications with urea. Unfolded ferritin was refolded by gel filtration chromatography with various concentration of urea. 50 mM Na-phosphate(pH 7.4) buffers with 2 M to 6 M urea were used in GFC. Objective was to characterize the structure change with urea concentration. Molecular weight was determined using GF-HPLC and RP-HPLC was used to quantify the unfolded and refolded proteins. Also the activiy of ferrin was confirmed by iron-uptake reaction.
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| 저자 |
김형원, 김인호
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| 소속 |
충남대 |
| 키워드 |
Refolding;ferritin;chromatography |
| E-Mail |
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| 원문파일 |
초록 보기 |
