| 초록 |
Microbial CO-utilization has been reported with diverse microorganisms including aerobic, anaerobic bacteria, phototrophs and archaea. Citrobacter amalonaticus Y19 is a unique strain of class Enterobacteriaceae that can carry out water-gas shift reaction using CO. Furthermore, Y19 has been characterized for formate-mediated fermentative H2 production showing the existence of various hydrogenases. In this study, for the first time, engineering of Y19 based on recent genomic information was carried out to improve the CO-dependent H2 production activity. Recently, genomic information for Y19 revealed the existence of two different CooA (CooA-1 and CooA-X) proteins which are located at different loci with different size, 672 and 252 bp. Commonly, CooA is known as transcriptional regulator of carbon monoxide dehydrogenase (CODH) by binding CO. Therefore, to increase CO-dependent H2 production activity and clarify the functions of two distinct CooA proteins, each CooA was overexpressed, and then characterized. |
