| 학회 |
한국고분자학회 |
| 학술대회 |
2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 |
28권 2호, p.254 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Galactosylated Poly(ethylenimine) (Gal-PEI)-graft-poly(vinyl pyrrolidone) (PVP) as Hepatocyte-Targeting Gene Carrier |
| 초록 |
The use of non-viral gene carrier such as cationic polymers or cationic lipids may overcome the current problems associated with viral gene carrier in safety, immunogenicity and mutagenesis. In this study, polyethylenimine (PEI) was used as a gene carrier and conjugated with lactose bearing galactose group for hepatocyte targeting and grafted with poly(vinyi pyrrolidone) (PVP) as a hydrophilic group to reduce cytotoxicity of PEI. The Galactosylated (Gal) PEI-PVP (Gal-PEI-PVP)/DNA complex was confirmed using agarose gel electrophoresis. The sizes and zeta potential of the complex were measured by dynamic light scattering. The cytotoxicity of Gal-PEI-PVP in HepG2 cells was evaluated. The Gal-PEI-PVP/DNA complex was transfected into HepG2 cells having asialoglycoprotein receptors (ASGRs), indication of specific interaction between ASGRs on HepG 2 cells and galactose ligands on Gal-PEI-PVP |
| 저자 |
국승은1, 박인규1, 조명행1, 김은미2, 정환정2, T.Akaike3, 조종수4
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| 소속 |
1서울대, 2원광대, 3Tokyo Institute of Technology, 4Japan |
| 키워드 |
gene delivery; PEI; PVP; Lactose; non-viral vector
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| E-Mail |
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