| 초록 |
We described a rapid, sensitive, and selective method for the colorimetric detection of biothiols in human serum by utilizing silver nanoprisms (AgNPRs) as the key detection components. In the absence of the target biothiols, the AgNPRs are etched by chloride ion in human serum, resulting in the yellow color that is indicative of silver nanoparticles (AgNPs). However, in the presence of the target biothiols such as homocysteine (Hcy), cysteine (Cys), or glutathione (GSH), the thiol groups of the target biothiols bind to the surface of AgNPRs via covalent interaction of Ag-S and protect the AgNPRs from being etched, thus exhibiting the blue/purple color which is indicative of AgNPRs. Importantly, we discovered that individual biothiols exert not only the anti-etching effect but also the aggregating effect on AgNPRs, which can be modulated by simply changing the pH conditions, and this enables the selective detection of each biothiol. Consequently, we successfully determined the Hcy, Cys, and GSH in human serum with high sensitivity and selectivity within 10 min, demonstrating the diagnostic capability and the potential for practical applications. |
