| 초록 |
This study was conducted to investigate the optimum culture condition for protease production by Enterobacteriaceae sp. In previous studies, Yeast extract, and TritonX-100 were identified as the significant factors affecting protease from one-factor-at-a-time method. The medium composition for cold-adaptive protease production of Enterobacteriaceae sp. was optimized by response surface methodology (RSM). RSM studies for optimizing protease production of Enterobacteriaceae sp. have been carried out for three parameters including yeast extract concentration, TritonX-100 concentration, and culture pH. These significant factors were optimized as 6.690 g/L yeast extract, 0.018 g/L Triton™ X-100, and pH 6.677. The experimentally obtained protease activity was 8.03 U /L, and it became 1.5-fold increase before optimization. |
