| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Expression of Recombinant Proteins Containing Unnatural Amino Acids in the AGGA Codon |
| 초록 |
Adding new amino acids into the set of building blocks for protein synthesis expands the scope of protein engineering. Orthogonal pairs of tRNA and aminoacyl tRNA synthetase have been developed to incorporate unnatural amino acids (UAAs). In particular, the tyrosyl-tRNA and tyrosyl-tRNA synthetase pair from Methanococcus jannaschii has been engineered for incorporating diverse UAAs into the amber codon. We were interested in taking advantage of the system to incorporate UAAs into four-base codon such as AGGA because the Mj tyrosyl-tRNA synthetase had been reported to have promiscuity toward anticodon. In this presentation, we show that the engineered Mj tyrosyl-tRNA synthetases activated MjtRNAUCCU without any additional engineering toward the tRNA anticodon and were used to incorporate UAAs quantitatively in response to the AGGA codon. In addition, the expression efficiency of recombinant proteins containing UAAs was improved by using engineered E. coli strain and the MjtRNAUCCU. |
| 저자 |
김수연, 유태현
|
| 소속 |
아주대 |
| 키워드 |
unnatural amino acid; four-base codon; tRNA
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| E-Mail |
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