| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 봄 (04/10 ~ 04/12, 부산컨벤션센터(BEXCO)) |
| 권호 |
44권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Enzyme Activatable Fluorogenic Hydrogel Lens Sensor |
| 초록 |
Hydrogels have offered biological applications such as scaffold, implantable devices and bio-sensors due to their advantages including (i) easy bio-functional modification, (ii) high biocompatibility, and (iii) prevention of non-specific adsorptions. Inflammatory enzymes, such as MMP-9, have been investigated widely because of their roles in development of inflammation. MMP-9 cleaves specific peptide sequences during degradation of extracellular matrix. Herein, we developed MMP-9 activatable fluorogenic hydrogel intraocular lens sensor using MMP-9 targetable peptide probe. In detail, C-terminus of peptide probe was conjugated inside the PEGDAAm-co-PAlAm hydrogel by EDC/NHS chemistry. PEGDAAm hydrogel showed improved stability compared to PEGDA hydrogels. Peptide probe inside the hydrogel sensor was cleaved with existence of MMP-9, becoming fluorescence off-state into on-state by detachment of dye and quencher. Fluorescence intensity was increased with concentration of MMP-9 increased. |
| 저자 |
신무광, 지용우, 강병훈, 문채은, 진우석, 기지선, 이효, 문병걸, 이형근, 함승주
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| 소속 |
연세대 |
| 키워드 |
하이드로젤; 안내렌즈; 바이오센서; 효소
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| E-Mail |
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