| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.428 |
| 발표분야 |
생물화공 |
| 제목 |
Extracellular Secretion of Cellobiohydrolase 1 from Trichoderma harzianum in Pichia pastoris by Using Heterologous Expression and Revealability of Its Cellulolytic Enzyme Activity |
| 초록 |
Trichoderma harzianum is a filamentous fungus that produces and secretes extracellular hydrolytic enzymes used in plant cell wall degradation. In this report, we identified a major unknown cellulase from T. harzianum and investigated characteristics of the cellulase using heterologous expression. One enzyme which had an exoglucanase activity was purified from T. harzianum through anion exchange chromatography. The purified enzyme revealed that it was cellobiohydrolase 1 (CBH1). Open reading frame of the cbh1 gene was cloned in the expression vector pPICZαA and transformed into Pichia pastoris X-33. The CBH1 was expressed in BMMY medium and the specific activity was displayed against Avicel of 0.07 U/ml. Recombinant cellobiohydrolase1 (rCBH1) was purified from the culture by Ni-NTA affinity chromatography and the purity of the enzyme was checked by SDS–PAGE. Further studies were investigated for characterization of rCBH1 enzyme activity. Our results revealed the cellulolytic enzyme activity of rCBH1 and secreted rCBH1 relevant to biofuel production. |
| 저자 |
박성현, 김수정, 전상덕, 한성옥
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| 소속 |
고려대 |
| 키워드 |
Cellobiohydrolase 1; Trichoderma harzianum; Heterologous expression; Pichia pastoris
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| E-Mail |
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| 원문파일 |
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