| 초록 |
Mussel adhesive proteins have been generally regarded as promising bioadhesives thanks to their fascinating properties including strong and flexible adhesion in any substrates in wet environments. Especially, fp-5 among mussel adhesive proteins has been considered a powerful bioadhesive from the highest DOPA content, although it was difficult to obtain high amount by natural extraction and recombinant approaches. In this study, we over-expressed Mytilus galloprovincialis-originated fp-5 in Escherichia coli and efficiently purified the protein using affinity chromatography. Bulk adhesion ability of fp-5 as a bioadhesive in medical or water environment was firstly determined from mass-production of fp-5, and its mechanical characteristics were also investigated. From this study, we expect fp-5 can be used as a significant bioadhesive material and an efficient multi-functional surface coating material. |
