| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 가을 (10/24 ~ 10/26, 부산 BEXCO) |
| 권호 |
18권 2호, p.1876 |
| 발표분야 |
생물화공 |
| 제목 |
Expression and Purification of Recombinant Spider Silk Proteins |
| 초록 |
The spider silks, especially the dragline and the flagelliform silk proteins arouse interests in these days because of excellent mechanical properties. The spider silks have highly repeated amino acid sequences which are composed with several amino acid motifs so that it is hard to express in Escherichia coli. We redesigned sequences of the spider silks and expressed the dragline silk (estimate MW is 26.9 kDa) and the flagelliform silk (estimate MW is 25.2 kDa) successfully in E. coli system. The expressed recombinant silk proteins were purified by immobilized metal affinity chromatography with his-tag. In addition, we present structure-activity relationship of the spider silks to suggest the way of prediction of protein structure and function from amino acid sequences. |
| 저자 |
권윤경1, 정두엽1, 양윤정1, 최유성2, 차형준1
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| 소속 |
1포항공과대, 2전북대 |
| 키워드 |
biomimetics; silk-like; recombinant protein; spider silk protein; molecular biotechnology
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| E-Mail |
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| 원문파일 |
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