| 초록 |
When it comes to shapes, mostly bacteria have the rod-like shape, whereas red blood cells (RBCs) have the disk-shaped structure. This research was focused on detecting bacteria exclusively. We investigated dependence on the shape by using light scattering in flow cytometry. Two different materials were used representing bacteria and RBCs. One is halloysite nanotubes (HNTs) having the rod-like shape. The other is laponite forming disk-shaped crystals in the water. The mixtures with different concentrations of HNTs were measured by flow cytometer for forward scattered signals. As increasing the concentration of HNTs, more events with lower intensity were counted. Isotropic laponite has the round-shaped scattering pattern keeping most scattered signals within the detecting area which can collect signals. In contrast, anisotropic HNTs form two symmetric lobes when scattered getting signals escape the area. Hence, the results suggest the possibility to distinguish between RBCs and bacteria. |
