| 초록 |
This study introduces a mechanically reinforced mussel-mimetic skin tissue sealing system in which a composite hydrogel bioadhesive consisting of bacterial cellulose nanofibers (BCNFs) and boronic acid-conjugated alginate (Al-BA) was employed. Boronic acid has a cis-diol moiety that exhibits pH-responsive curing from a viscoelastic solution to a soft gel under physiological conditions. However, Al-BA itself has a limitation to tissue suturing due to formation of intrinsic soft gel phase. Therefore, it is necessary to develop a technology to reinforce the gel phase which has suitable adhesion strength for tissue sealant. For this, we conjugated 3-aminophenylboronic acid onto an alginate backbone via EDC/NHS-mediated reaction, and then hybridized with BCNFs. Finally, we demonstrated that our bioadhesives exhibited effective skin tissue sealing even under wet conditions, thus promising a variety of biological applications in the field of tissue engineering. |
