| 초록 |
Developing accurate and sensitive bioassays for various point-of-care applications are important issues. In this work, Fe3O4-Pt/core-shell nanoparticles (MPt/CS NPs) were synthesized for fulfilling such demands and the bioassay based on unique property of the newly synthesized nanozymes. Fe3O4 has its own catalytic activity superior to that of natural enzymes, but incorporating Pt to its outer layer of the Fe3O4 can improve the catalytic activity while building heterogeneous nanostructures. Catalytic properties of MPt/CS NPs allow signal amplification by enzyme-like reactions. Furthermore, magnetic properties of MPt/CS NPs enable magnetic enrichment of liquid samples. By integrating MPt/CS NPs in lateral flow immunoassay strips, one of the widely used point-of-care bioassay devices, and harnessing magnetic and enzyme-like properties of MPt/CS NPs, an increase in sensitivity of two orders of magnitude was achieved compared to the sensitivity of conventional lateral flow immunoassay based on Au nanoparticles. |
