| 초록 |
To specially comprehend the function and selective evolution of a cell, its compartmentalization has attracted much interest in recent years owing to the characteristic appearance and intrinsic regulatory system. Here we first present a pseudo-eukaryote-nucleus (PEN) which is able to function as a transcript decoder for an enhanced mRNA level. It is simply created by enzymatically knitting a genome with histone-like DNA block crosslinkers. After further processing of lipid encapsulation and successive addition of transcription factors, it promoted the controllable expression of mRNA. In comparison with other control groups (e.g., nucleus-free system), it exhibited the increment of green fluorescent protein (GFP) mRNA tested. As being internalized by target cell (e.g., MCF-7), it resulted in higher and longer expression of GFPs. |
