| 학회 |
한국재료학회 |
| 학술대회 |
2021년 가을 (11/24 ~ 11/26, 경주 라한호텔) |
| 권호 |
27권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Studies of photo-reversible fluorescent hydrogel with pdDronpa: Synthesis and characterization |
| 초록 |
Dronpa145N is a tetrameric fluorescent protein undergoes reversible photo-switching and photo-dissociation by cyan light (dissociation) and violet light (re-association). pdDronpa is a dimeric derivative of Dronpa145N which is bright and more sensitive to light while forms less aggregates both in vitro and in vivo. We synthesized light-controllable fluorescent hydrogel with polyethyleneglycol (PEG)-maleimide and pdDronpa. This hydrogel showed reversible gel-sol transition under cyan light and violet light. Then, we added restriction enzyme-treated lambda DNA fragments during hydrogel synthesis. After cyan light illumination, DNA fragments were able to escape from the hydrogel, and DNA chelating dye could bind to them resulting in strong red fluorescence. |
| 저자 |
김연지, 김남두
|
| 소속 |
공주대 |
| 키워드 |
<P>pdDronpa; Hydrogel</P>
|
| E-Mail |
|
