| 초록 |
Versatile tumor models are available to study cancer biology and to screen anti-cancer drugs. In the most simplistic approach, two-dimensional (2D) tumor culture models lack realistic complexity, while animal models are expensive, and time consuming. So, three-dimensional (3D) culture have increasingly used for a wide variety of fundamental researches and therapeutic applications. However, hydrogels, such as collagen hydrogel or Matrigel, do not reflect the heterogeneity of tumor microenvironment in patients. Here, using patient-derived extracellular matrix (pECM) and patient-derived glioma cancer cell (pGC), we reconstructed microtumors. By using the pECM, we investigated cell morphology, invasion, gene expression and drug response. Our results revealed that pGCs exhibit different characteristics in various ECM. Thus, we suggest that tumor- and patient-specific 3D tumor models could provide a better understanding in tumor biology and guide to choose personalized therapeutic options. |
