| 초록 |
Cost-effective biosensors are drawing great attention for sensing significant neurotransmitter dopamine (DA). Herein, we developed the laccase immobilized fiber-optic biosensor thorough fluorescence mechanism for sensing DA. We employed microwave reaction for preparing carbon dots (CDs) using curcumin and dimethylformamide (CDD-CDs). Further, CDD-CDs were silica functionalized through 3-(aminopropyl)-triethoxysilane, a silicon precursor (APT-CDs). Laccase was covalently immobilized to APT-CDs (i.e., bioprobe). CDD-CDs, APT-CDs, and bioprobe displayed orange, green, and blue-colored emissions. Bioprobe delivered fluorescence quenching against DA with a detection limit of 41.2 nM in concentrations (0-30 μM). Bioprobe was immobilized on the tapered optical fiber by ethyl cellulose using a facile dip-coating. Tapered optical fiber showed a detection limit of 46.4 nM for DA in concentrations (0-10 μM). Bioprobe showed multi-color imaging behavior in human neuroblastoma cells (SH-SY5Y). |
