| 초록 |
Dip-pen nanolithography (DPN) is one technique that has shown particular promise in this area, allowing one to prepare standardized multi component arrays of biomolecules that can retain their biorecognition properties once transferred to a surface. The method has been used to create nanometer-scale patterning based on small organic molecules as well as biological macromolecules such as proteins, oligonucleotides, peptides, and viruses. The aim of this work is to fabricate the E.coli bacteria micro arrays via DPN methods on LB-agar substrate by using poly(2-methyl-2-oxazoline)(PMeOx)-coated AFM probe which is fabricated by ring opening polymerization of 2-methyl-2-oxazoline monomers on the probe surface. In addition, we demonstrate that the patterned bacterial cells can be proliferated on LB-agar substrate on the appropriate incubation conditions. The synthesized PMeOx-coated AFM probe and pattern of bacterial cells were characterized with fluorescent microscope, SEM and AFM. |
