| 학회 | 한국재료학회 |
| 학술대회 | 2018년 가을 (11/07 ~ 11/09, 여수 디오션리조트) |
| 권호 | 24권 2호 |
| 발표분야 | 6. 의료기기 핵심 뿌리기술 심포지엄(Symposium on material science for biomedical device)-오거나이저: 김현종 박사(KITECH) |
| 제목 | Highly Sensitive Non-Enzymatic Lactate Biosensor based on Nickel Oxide derivatives |
| 초록 | The emerging need for human health monitoring using wearable devices has drawn considerable attention to the detection and continuous monitoring of metabolites in body fluids such as blood and perspiration. Among various metabolites, the concentration of lactate is considered as a key parameter to monitor tissue oxidation in clinical diagnositics. So far, most research in the development of amperometric lactate biosensors has focused on enzymatic sensor that is electrode treated with lactate-active enzyme due to its biological selectivity. Despite this advantage, enzyme-immobilized biosensor has still suffered from the short-term stability because of loss of enzyme activity over time induced by biofouling and biodegradation.[1] Alternatively, non-enzymatic lactate sensor has been proposed employing earth-abundant materials that are biologically stable as well as electrochemically active to lactate. However, the lower sensitivity of non-enzymatic approach than enzymatic one is still problematic, thus should be addressed in order to bring it to commercialization. In this presentation, we demonstrate a highly sensitive non-enzymatic lactate biosensor based on nickel oxide derivatives, NiO and Ni(OH)2. We assessed the capabilities of NiO and Ni(OH)2 for lactate sensing in terms of sensitivity, selectivity and response time. Electrochemical and materials characterizations revealed the difference in sensing mechanism of NiO and Ni(OH)2, suggesting that the NiO electrode provided faster and more reliable lactate sensing than Ni(OH)2.[2] In addition, the high sensitivity of NiO based lactate sensor are achieved using porous nickel oxide nanostructure as providing more pathways for direct electrons transfer between the redox stages and electrode surface.[3] Porous nanostructure of nickel oxides are fabricated employing sol-gel based inverse micelle method with different calcination temperature from 250℃ to 450℃ in order to control microstructural characteristics. Lactate sensitivity of nickel oxides with different porosity are also assessed using cyclic voltammetry (CV). It offered a wide dynamic detection range of 0.01-27.76mM as well as selective detection of lactate in the presence of several interferences such as ascorbic acid, dopamine and uric acid. Furthermore, the proposed electrodes also exhibit high sensitivity of 80.763 µA/mMcm-2, low detection limit of 0.42 mM, respectively, which are comparable to those of enzymatic lactate sensors reported previously. [4] In addition, we also explored microstructural and chemical characteristics with different calcination temperature using various characterization methods such as Brunauer-Emmett-Teller (BET), FT-IR, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD), thus revealed the origin of best performance in nickel oxide. [1] G. Rocchitta, A. Spanu, S. Babudieri, G. Latte, G. Madeddu, G. Galleri, S. Nuvoli, P. Bagella, M. I. Demartis, V. Fiore, R. Manetti, P. A. Serra, Sensors, 2016, 16, 780. [2] S. Kim, K. Kim, H-J. Kim, H-N Lee, T.J. Park and Y.M. Park Electrochimica Acta 2018, 276, 240 [3] M. Etienne, L. Zhang, N. Vilà, A. Walcarius, Electroanalysis, 2015, 27, 2028. [4] K. Rathee, V. Dhull, R. Dhull, S. Singh, Biochem. Biophys. Rep., 2016, 5, 35 |
| 저자 | 박영민 |
| 소속 | Korea Institute of Industrial Technology |
| 키워드 | Lactate detection; Nickel oxide; Nickel hydroxide; Electrochemical biosensor; Amperometric biosensor |
