| 초록 |
Recently, microRNAs (miRNAs) have been widely recognized as biomarkers of various diseases, especially cancer, which led to active research for sensitive and selective microRNA detection systems. We herein describe an enzyme-free miRNA detection method which employ catalytic hairpin assembly (CHA) and DNA-templated silver nanoclusters (AgNCs) for fluorescence enhancement. This strategy, termed “CHA-guided activation of AgNCs by G-rich DNA (CHA-AG)”, utilizes two hairpin DNA probes, HP1 and HP2, which include silver nanocluster template and G-rich DNA overhang sequence, respectively. Target-triggered formation of HP1-HP2 complex can emit highly enhanced fluorescence signal at the wavelength of 634nm. Using this strategy, highly sensitive and selective detection of several miRNAs was demonstrated in the dynamic range of 0.01-200nM. Due to the ease of manipulating fluorescence emission wavelengths by simply changing the AgNC template sequence, it is expected that this work can be further expanded for multiplex miRNA detection. |
