| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 봄 (04/27 ~ 04/29, 부산 BEXCO) |
| 권호 |
22권 1호, p.530 |
| 발표분야 |
생물화공 |
| 제목 |
Preparation of Fe3O4@Nickel-Silicate Core-Shell Nanoparticles for Immobilization of His-Tagged Enzymes |
| 초록 |
Enzyme immobilization technique provides adequate means to not only efficient use of proteins but also easy-remove of product contamination. Immobilization of enzyme on inorganic nanoparticles have been widely investigated due to the benefits of large surface area and high stability in various temperature. However, currently applicable method to synthesize transition metal ions on a surface of nanoparticles requires complicated steps. Herein, we synthesized spiky shaped Fe3O4@nickel-silicate core-shell nanoparticles via facile synthetic route. Nickel ion surfaced nanoparticles exhibited high immobilization capacity (80ug/mg) of His-TEV protease, bound based on His-tag and transition metal ion affinity. To investigate enzymatic activity of immobilized TEV protease, we analyzed cleaved quantity of MBP-IgG protein, which have TEV protease-specific cleavage site. Finally, these enzyme immobilized nano-complex were separated from reactant and reused by application of external magnetic field. Consequently, our newly developed nickel doped nanoparticles would provide several advantageous features in nanoparticle-based enzyme immobilization techniques. |
| 저자 |
신무광1, 강병훈1, 기지선1, 한승민1, 김명훈1, 윤남경2, 안정오2, 함승주1
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| 소속 |
1연세대, 2생명공학(연) |
| 키워드 |
효소/단백질공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
