| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Precise flux redistribution by exploiting glyoxylate cycle for efficient 5-aminolevulinic acid production |
| 초록 |
Microbial production of 5-aminolevulinic acid (ALA) has received much attention because of its potential in clinical applications. The aim of this study was precise carbon flux optimization for high ALA production in Escherichia coli expressing the ALA biosynthetic pathway. Initially, genes hemA and hemL were overexpressed with strong promoters and synthetic 5′-untranslated regions (5′-UTRs). Then, the tricarboxylic acid (TCA) cycle was blocked to force carbon flux toward the ALA production pathway by deletion of sucA. Although the resulting strain showed a severe metabolic imbalance and low ALA production, further precise tuning of carbon flux to the glyoxylate cycle by varying the transcriptional strength of aceA led to substantially improved cell growth and ALA production. Thus, this precise tuning of the glyoxylate cycle in a quantitative manner should also enable efficient production of other value-added products derived from the TCA cycle. |
| 저자 |
노명현1, 임현규1, 박성훈2, 서상우3, 정규열1
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| 소속 |
1POSTECH, 2UNIST, 3서울대 |
| 키워드 |
5-Aminolevulinic acid; Glyoxylate cycle; Metabolic engineering; Synthetic biology
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| E-Mail |
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