| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Efficient engineering for tyrosine production from acetate in E. coli |
| 초록 |
Acetate is one of promising feedstocks owing to its cheap price and great abundance. Here, we engineered a previously reported strain, SCK1, for efficient production of tyrosine from acetate. Initially, the acetate uptake and gluconeogenic pathway were amplified to maximize the flux toward tyrosine. As flux distribution between glyoxylate and TCA cycles is critical for efficient precursor supplementation, the activity of the glyoxylate cycle was precisely controlled by expression of isocitrate lyase gene under different-strength promoters. Consequently, the engineered strain with optimal flux distribution produced 0.70 g/L tyrosine with 20% of the theoretical maximum yield which are 1.6-fold and 1.8-fold increased values of the parental strain. This is the first demonstration of tyrosine production from acetate. Our strategies would be widely applicable to the production of various chemicals from acetate in future. |
| 저자 |
조민지1, 노명현1, 임현규1, 강채원1, 임대균2, 오민규2, 정규열1
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| 소속 |
1포항공과대, 2고려대 |
| 키워드 |
Tyrosine; glyoxylate cycle; acetate
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| E-Mail |
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