| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Preparation and the Skin Permeation Characteristics of rhEGF (recombinant human epidermal growth factor) loaded Nano-liposome |
| 초록 |
recombinant human epidermal growth factor (rhEGF) was encapsulated into nano-liposomes (NLs) system for topical delivery. The rhEGF-loaded NLs were prepared using a high pressure homogenization method. Morphology and overall particle distribution of NLs were investigated using transmission electron microscopy (TEM) and high resolution microscope (CytoVivaTM). Particle size, zeta (z) potential and encapsulation efficiency were measured and the percutaneous delivery of NLs was evaluated using Franz diffusion cells and immunofluorescence confocal laser scanning microscopy (CLSM). The mean particle size, z potential and encapsulation efficiency of the NLs were 155.57±2.59 nm, -57.92±4.35 mV and 9.00±0.39%, respectively. TEM and microscopic analysis showed spherical, very even-sized vesicles approximately 150 nm. The skin permeation and localization of rhEGF were enhanced by NLs. CLSM image analysis provided that the NLs enhanced the permeation and localization of rhEGF in rat skin by facilitating entry through pores of skin. |
| 저자 |
전상옥1, Santosh Bashyal2, 서조은2, 오동호1, 이상길2
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| 소속 |
1인제대, 2계명대 |
| 키워드 |
recombinant human epidermal growth factor; nano-liposome; percutaneous delivery; skin localization
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| E-Mail |
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