| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Translocation mechanism of poly(ethylene glycol)-coated nanoparticles into brain: the role of apolipoproteins on receptor-mediated endocytosis. |
| 초록 |
Poly(methoxy poly(ethylene glycol)-co-hexadecyl cyanoacrylate (PEG-PHDCA) nanoparticles have demonstrated their capacity to diffuse through the blood-brain barrier (BBB) after intravenous administration. However, the transport mechanism of these nanoparticles into brain was not yet clearly elucidated. Thanks to the establishment of the primary culture of rat brain endothelial cells (RBEC), investigations into mechanism became accessible. Intracellular distribution and internalization experiments of nanoparticles in RBEC showed that this passage occurred via a specific endocytosis. The inhibition of nanoparticle endocytosis by chlorpromazine and by the anti-low density lipoprotein receptor (anti-LDLR) antibody and at the opposite the increase of cell uptake of ApoE- and ApoB-100-preincubated nanoparticles demonstrated that the clathrine-dependant and LDLR-mediated pathway was involved in the internalization of PEG-PHDCA nanoparticles. The adsorption of ApoE and/or ApoB-100 onto the surface of PEG-PHDCA nanoparticles in serum was also revealed as the first step of the mechanism allowing their recognition by LDLR in the RBEC membrane and leading to their endocytosis. The design of new and more efficient colloidal carrier for brain targeting will be based on the comprehension of this mechanism. |
| 저자 |
Hyun Ryoung KIM1, Karine ANDRIEUX2, Patrick COUVREUR3
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| 소속 |
1Laboratoire de Physico-chimie, 2Pharmacotechnie et Biopharmacie, 3UMR CNRS 8612 |
| 키워드 |
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| E-Mail |
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