| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
고분자_포스터 |
| 제목 |
Microchip Capillary Electrophoresis Device for High-Resolution SSCP Analysis Using Triblock Copolymer Matrix |
| 초록 |
Accurate, cost-effective and fast genetic marker detection method is required for the molecular genetic diagnosis. Microchip Capillary electrophoresis-single strand conformation polymorphism (μCE-SSCP) is has great potential for genetic variation detection because the method can discriminate differences in conformation of single-strand DNA molecules even similar sized molecules with difference sequence. Only heat denaturing and fast cooling step is required for the formation of single-strand conformer. However the most critical limitation of μCE-SSCP is low separation resolution result from short separation channel length. In our previous research, PEO-PPO-PEO triblock copolymer matrix was demonstrated that significantly increase the separation resolution on bench-top CE-SSCP. The limitation of μCE-SSCP could be overcome by utilizing the triblock copolymer matrix. As a result, multiplex and quantitative genetic analysis could be possible to realize on CE-SSCP based microchip device for early stage fast and sensitive molecular diagnosis. |
| 저자 |
정규열, 신기영
|
| 소속 |
포항공과대 |
| 키워드 |
CE-SSCP; microchip; molecular diagnosis
|
| E-Mail |
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