| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 | 15권 2호, p.1607 |
| 발표분야 | 생물화공 |
| 제목 | Sensitive detection of human cancer-related SNPsusing high resolution CE-SSCP analysis system |
| 초록 | Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) analysis is known as a simple and rapid molecular marker detection technique such as detections of single nucleotide polymorphisms (SNPs). Due to its high sensitivity and great reproducibility with conformation dependent separation of single strand DNA, CE-SSCP shows great capability for detecting SNPs. However, conventional polymer matrices generally used for CE-based analysis has poor resolution in CE-SSCP since it is optimized for molecular weight dependent separation. In this study, we introduced new polymer matrix for high resolution CE-SSCP analysis. The model systems were SNP sites of human cancer-related genes which were frequently detected on cancerous tissues. To improve resolution, various PCR amplicons that including target SNP sites were designed, which were varied in length, relative SNP position, and fluorescent dye position. The results showed that SNPs detection using newly introduced polymer matrix could detected SNPs much more sensitively when compared with conventional homopolymer matrix. |
| 저자 | 황희성, 신기원, 정보람, 정규열 |
| 소속 | 포항공과대 |
| 키워드 | CE-SSCP; SNP; molecular diagnosis |
| 원문파일 | 초록 보기 |
