| 초록 |
In this presentation, I introduce plasmon based nanoscale sampling methods to break a diffraction limit with nanostructures in total internal reflection fluorescence (TIRF) microscopy. Various fluorescent molecular targets, such as adenovirus, microtubules and proteins, were used to measure the imaging resolution for a broad application. Significant resolution improvements were achieved down to 70 nm imaging resolution without fundamental deviation from a conventional TIRF microscope. I compared those performances to an extraordinary transmission based axial imaging (EOT-AIM) technique using linear nanoaperture arrays. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide-field microscopy for acquisition of lateral images. A current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. |
