| 학회 | 한국재료학회 |
| 학술대회 | 2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 | 22권 2호 |
| 발표분야 | A. 전자/반도체 재료 분과 |
| 제목 | Investigating Optogenetically Induced Absence Seizure through Flexible Multi-Electrode Array |
| 초록 | Establishing the neural dynamics and operating mechanism of the brain is crucial for medical analysis. Especially, optogenetics is an updated tool which controls and investigates specific neural circuits with light and light-sensitive proteins within neuron. Compared with electrical stimulation, optogenetics has relatively high resolution and is able to realize in-depth study about neuronal circuitry by activating targeted neuronal populations. In this case, light-sensitive proteins called opsins such as Channelrhodopsin 2 (ChR2) and Halorhodopsin (NpHR) are injected into the certain cells, which can be activated by ~470 nm and ~580 nm wavelength of light, respectively. Normally, optogenetics consist of opsins, light sources and recording techniques. To assess the neural network by light stimulation, it is important to read out the responsible signals from neurons. However, conventional tools including patch clamp have drawbacks as momentary or narrow analysis and tissue damage. These problems can be solved by using flexible electrode, because the recording device strongly depends on the interfacial properties between the electrodes and brain. In particular, flexible multi-electrode array is appropriate for improving electrochemical stability, biocompatibility, conformal coverage and broad area investigation for real time, permanent and specific analysis of neural dynamics. Here we show the flexible micro electrode array applied to cortical surface directly as electrocorticogram(ECoG) recording tools, which is accompanied with optical fiber for light stimulation. By exploiting this device, systematic cortical responses from optogenetic inhibition of mouse ventral thalamus were investigated, enabling us to observe widespread absence seizure signals from large cortical area, which would be impossible with focal area recording techniques. Utilizing flexible electrode with optogenetics may be effective on interpreting previous studies using optogenetic tools, providing us new insights on how information transferred in our brain. |
| 저자 | 신정호, 이승현, 이한얼, 이건재 |
| 소속 | 카이스트 |
| 키워드 | Flexible; Electrode; Optogenetic |
