| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
(생체재료) Smart Responsive Biomaterials |
| 제목 |
Hydrogel-laden Substrates for Origami-based Tissue Engineering |
| 초록 |
In this study, we present a novel method to assemble biofunctionalized paper into 3-dimensionally (3D) structured complex scaffold system for reliable tissue regeneration through origami-based method. The surface of paper scaffold was conformally modified with poly(styrene-co-maleic anhydride) (PSMa) layer via initiated chemical vapor deposition (iCVD) followed by immobilization of poly-L-lysine (PLL) and Ca2+. This procedure ensures the gelation of a thin cell laden-alginate hydrogel film strongly adhesive on the paper substrate due to the diffusion of Ca2+ to form electrostatic interaction between the hydrogel film and PLL. The developed scaffold system was highly versatile, allowing area-selective cell seeding, which enabled the hydrogel-cell laden paper scaffold to achieve optimal biological cues for the regeneration of desired tissue. In addition, the paper-based scaffold can be folded freely into 3D tissue-like structures by simple origami-based method. Hydrogel-chondrocyte-laden cylindrical shaped paper origami was transplanted into three-ring defect trachea in rabbit for tracheal reconstruction. Transplanted engineered tissues replaced the native trachea without stenosis after 4 weeks. As for the custom-built scaffold system, the origami-based 3D scaffold system will provide a robust and versatile method to form complex tissues mimicking native tissue constructs. |
| 저자 |
황석연 |
| 소속 |
서울대 |
| 키워드 |
Paper scaffolds; origami; tissue engineering; hydrogel; initiated chemical vapor deposition
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| E-Mail |
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