| 초록 |
In this study, we propose a mechanically robust lipid nanovesicle system as an effective drug delivery nanocargo during the blood circulation with enhanced cellular uptake in many diseases. We controlled the rigidity of nanovesicles structured with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) by coassembling with amphiphilic poly(ethylene oxide)-block-poly(ε-caprolactone)-block-poly(ethylene oxide) (PEO-b-PCL-b-PEO). We observed that the presence of PEO end blocks increased the longevity of nanovesicles during blood circulation due to the anti-fouling effect, and the PCL middle block with high crystallinity make the membrane rigid. The stiffness of nanovesicles was analyzed by using quartz crystal microbalance with dissipation (QCM-D). Thanks to mechanical strength, it is expected that highly stiff nanovesicles remain intact in the bloodstream and effectively penetrate into the target cell suggesting its clinical potential. |
