| 초록 |
A rapid and efficient strategy to detect Vascular Endothelial Growth Factor (VEGF) as cancer biomarker was developed with field-effect transistor (FET) sensor based on carboxylated polypyrrole nanotubes (CPNTs). The CPNTs used as high performance transducers of this FET system were successfully fabricated by cylindrical micelle templates in a water-in-oil emulsion system. On the basis of CPNTs, a FET sensor platform was constructed to detect specific biological entities by using a buffer solution as a liquid-ion gate. The CPNTs were covalently immobilized onto the microelectrode substrate to make a good electrical contact with metal electrodes, and anti-VEGF RNA aptamer as the molecular recognition element was bonded to the nanotube surface via through condensation reactions in the liquid phase. Importantly, the VEGF detection limit of the FET sensor based on CPNTs conjugated with anti-VEGF RNA aptamer (CPNTs-aptamer) was found to be near 400 fM in real-time. |
