| 초록 |
Detection of protein biomarkers is important for diagnosing human diseases and pathological conditions. Methods such as HPLC, ELISA, and Western blot are widely used in research laboratories, however show limitations for applications in point-of-care testing. Herein, we introduce a label-free sensing method using carbon dot nanoparticles with fluorescence which can be switched on and off depending on presence of the protein target. The assay is performed by mixing the carbon dot solution with dopamine, along with the fluid sample including the target. The formation of polydopamine results in quenching of the fluorescence from the carbon dots, while the addition of a target protein results in a ‘positive’ fluorescence signal. We show that the method can be applied as a simple and rapid diagnostic assay by ubiquitous detection of various protein targets. |
