| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
22권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Label-free detection with ligand immobilization on SERS-encoded and polydiacetylene-coated beads |
| 초록 |
Multiplex protein detection technology is a powerful tool to investigating the interaction of protein and protein. Since the efficiency and convenience, encoded bead was usually used for this detection method. However, high-throughput detection of encode bead is still challenging because of the difficulty of binding between labeled secondary protein and target protein. In this study, we introduced biotin to the polydiacetylenecoated surface-enhanced Raman scattering-encoded beads (PDA–SERS beads) to detect target protein model (streptavidin) in the PDA-SERS bead system. The fluorescence intensities of biotin-conjugated PDA–SERS beads were increased by the increasing concentration of streptavidin. We could detect streptavidin with detection limit of 2ⅹ10-8 M throughout the fluorescence intensity without additional labeling step. |
| 저자 |
강은지, 함은일, 김형모, 하유나, 김태한, 전봉현
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| 소속 |
건국대 |
| 키워드 |
Surface enhanced Raman scattering; Polydiacetylene (PDA); Label-free detection
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| E-Mail |
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