| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 봄 (04/22 ~ 04/23, 여수대학교) |
| 권호 |
11권 1호, p.360 |
| 발표분야 |
생물화공 |
| 제목 |
Rapid purification system for His-tagged protein in a cell-free protein synthesis system |
| 초록 |
We present a rapid protein expression and purification method in a cell-free system. Overall process is a single step that is isolated His-tagged proteins during cell-free synthesis from a batch reaction. However, a higher proportion of other proteins may also bind to immobilized metal-ion affinity chromatography (IMAC) resin. We described the use of pre-treated S30 extract with same resin and washing buffer with a low concentration of imidazole(30 mM) for the removal of contaminants. The results document that it should be possible to purify with a higher purity and to overcome the above problems. Our method may allow a faster and more convenient approach to study of High-throughput technology compared to classical in vivo system. |
| 저자 |
김태완1, 오인석1, 금정원1, 최차용1, 김동명2
|
| 소속 |
1서울대, 2충남대 |
| 키워드 |
cell-free protein synthesis; protein purification; High-throughput technology
|
| E-Mail |
|
| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
