| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.876 |
| 발표분야 |
재료 |
| 제목 |
Nickel Ferrite (NiFe2O4) Nanoparticle Clusters for the Efficient Recyclable Separation of Histidine-Tagged Proteins |
| 초록 |
Affinity separation based on a specific interaction between a metal ion and histidine-tagged (His-tagged) protein is one of the most efficient methods for purifying recombinant proteins. In order to devise a simpler and more efficient method for the recyclable separation of His-tagged protein, nickel ferrite (NiFe2O4) nanoparticle clusters (NPCs) were synthesized by a simple hydrothermal method and employed in this work. The structure of NiFe2O4 NPCs provides a large surface area (105.0 m2 g-1) and pore volume (0.32 cm3 g-1), which are preferable for the separation of large amounts of proteins. The high magnetic saturation value (41.3 emu g-1) and superparamagnetic property of these materials lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were strongly maintained for up to 7 cycles. |
| 저자 |
천진녕, 서상우, 정규열, 이진우
|
| 소속 |
포항공과대 |
| 키워드 |
Nickel ferrite; Superparamagnetic nanoparticles; Histidine-tagged protein separation
|
| E-Mail |
|
| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
