| 학회 |
한국재료학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 광주 김대중컨벤션센터) |
| 권호 |
27권 1호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
Fabrication of Bi-layered small diameter vascular grafts by using electrospun Silk fibroin and PU/PCL nanofibers with mechanical property, in vitro and in vivo biocompatibility analysis |
| 초록 |
There is an increasing demand for functional small-diameter vascular grafts (diameter ＜6 mm) to be used in clinical arterial replacement. Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun (PCL/PU) polycaprolactone/Polyurethane (outer layer) and silk fibroin (inner layer) bi-layered scaffold (diameter ＜2mm) was produced. Scanning electron microscope (SEM) analysis was performed and the micrographs showed that the fibers of both layers were smooth and homogeneous. Water contact angle measurements further demonstrated hydrophilicity of the scaffold. Tensile test for mechanical strength was done on bi-layered scaffold to perceive appropriate properties for the specific application. To determine cell attachment and migration on the scaffold, outer layer was seeded with Rat Primary Glomerulus Endothelial Cell (rGEC) and inner layer was loaded with heparin and fibroblast growth factor (bFGF). MTT assay was showed that the cells grew and proliferated well on both layers of the bi-layered scaffold. Overall, these in vitro studies revealed that the bi-layered PCL/PU-Silk scaffold was biocompatible and had a good mechanical behavior. |
| 저자 |
Md. Abdullah Al Fahad1, Byong-Taek Lee2
|
| 소속 |
1Department of Regenerative Medicine, 2College of Medicine |
| 키워드 |
small diameter vascular grafts; electrospinning; PCL; silk; PU
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| E-Mail |
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