| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Optimization of scaffold and hydrogel-seeding method for vascular tissue engineeering |
| 초록 |
This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated by an extrusion-particulate leaching method using a biodegradable elastic polymer, poly(L-lactide-co--caprolactone) (PLCL) (50:50, Mn 1.58105). we polished the outer lumen of scaffolds with sandpapers to remove the closed pore for the improved infiltration of cells-hydrogel mixture into the inside of scaffold. In addition, we used a vacuum seeding method to infiltrate cell-hydrogel mixture into scaffolds. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes 50-100μm, 100-200μm, and 300-500μm, respectively. Especially, the cell proliferation efficiency improved by using the hydrogel-seeding method as compared with by using an established method. |
| 저자 |
박인수, 김상헌, 김수현, 한정우, 고영건, 정은나
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| 소속 |
한국과학기술(연) |
| 키워드 |
vascular tissue engineering; hydrogel; biodegradable elastic polymer; cell proliferation
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| E-Mail |
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