| 초록 |
To make clinically useful genotyping methods for single nucleotide polymorphisms (SNPs), the three factors which is accuracy, simplicity, sensitivity are important. One of the developed SNP genotyping technology is the ligation-dependent method which is the simplest method for clinical diagnosis. But the sensitivity is not ensured by itself, and analysis of various targets is limited by the detection method. To compensate the defect of ligation dependent method, we employed the ligase detection reaction (LDR) coupled with high-resolution CE-based single-strand conformation polymorphism (CE-SSCP) to develop robust SNP genotyping method. We found that it could precisely distinguish mismatches of bases and detect 37 SNPs of the tp53 gene quantitatively, which are used as targets in multiplex analysis, using three-color fluorescence-labeled probes. |
