| 초록 |
Generally, two different approaches have been utilized for the delivery of genes in gene therapy, namely that of viral vectors and non-viral delivery systems mainly using cationic polymers or lipids. Viral vectors show excellent transfection efficiencies. However, their use in clinical applications are often limited by several problems, including the potential of mutagenicity, oncogenesis, and several host immune responses. Non-viral systems, especially polymers, show significantly lower safety risks and can be tailored to specific therapeutic needs. However, the major disadvantage of these non-viral vectors is their low transfection efficiency compared by viral vectors. In this research, we developed multifunctional polymeric gene carriers such as biodegradable or bioreducible. Physicochemical properties and their transfection efficiency of polymer/gene complexes were investigated by using several cell lines. |
