| 초록 |
Chitosan has been widely used as a delivery carrier of DNA and siRNA due to its low toxicity, low immunogenicity, and positively charged amino groups. Small interfering RNA (siRNA) is a potential therapeutic for treatment of various diseases. However, the use of siRNA is limited due to its rapid degradation and low intracellular association. Chitosan-based nanoparticles encapsulating siRNA were prepared using a coacervation method in the presence of polyguluronate (PG). PG is isolated from alginate and is strongly related to ionic interactions of negatively charged alginate. Various physicochemical properties of chitosan/PG nanoparticles, including size, surface charge, morphology, and interaction with siRNA, were characterized. Nanoparticles showed low cytotoxicity and were useful in delivering siRNA to HEK 293FT and HeLa cells. |
