| 초록 |
Polycaprolactone(PCL) is a non-toxic aliphatic polyester with biodegradability and biocompatibility, which has a potential as promising bone substitutive materials because of its slow degradation properties. Hydroxyapatite(HA) which mimics the natural bone minerals has good osteoconductive properties and poor fabrication properties. Hyaluronan benzyl ester(HyA-B), which can be processed leading to a great variety of products without altering the biocompatibility and biological activity of hyaluronic acid(HyA), has been found to be suitable substrate to grow a variety of cell types. Therefore, porous biodegradable PCL/HyA-B/HA scaffolds were prepared to have the good mechanical and osteoconductive properties, and suitable surface for cell attachment and proliferation. HyA was modified with benzyl bromide to form HyA-B that was soluble in organic solvents. The synthesis of HyA-B was confirmed using FTIR and 1H-NMR. Also, porous biodegradable PCL/HyA-B/HA scaffolds were fabricated by a gas-foaming method using a combination of two effervescent salts, ammonium bicarbonate and citric acid. The compressive mechanical strength and morphology of these scaffolds were examined. The cell attachment and proliferation onto the scaffolds was examined by using human MG63 osteoblast cell. The resultant PCL/HA/HyA-B scaffolds have a well interconnected macroporous structure of pore size ranging from 150-200 ㎛. Human osteoblast cell culture showed that the proliferation increased with HA content in the scaffold. The synergy effect of HyA on the osteoblast proliferation was observed, too. |
