| 초록 |
Hilghly porous scaffolds of PLGA was prepared by thermally induced phase separation(TIPS). These scaffolds were loaded with plasmid DNA to achieve sustained release. A variety of parmeters were involved in the process, such as polymer concentration, solvent ratio, and quenching methods. The scaffolds with different internal structure could be formed by controlling the parameters of scaffolds fabrication. Pore structures of scaffolds showed wide variations depending on the process, especially, quenching method and aging time. Plasmid DNA loaded into scaffolds was released for 3 weeks in vitro. The release profile colud be controlled according to the pore structures and its connectivity. DNA release was hindered by blending PLL into polymer and the release kinetics could be controlled, too. Scaffolds loaded with DNA were implanted into mice and transfection of DNA into cells was observed. |
