| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 |
15권 1호, p.402 |
| 발표분야 |
생물화공 |
| 제목 |
Microfluidic enzymatic-reactors using carbon nitride supports for rapid protein identification |
| 초록 |
Protein identification through peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS) has become a standard technique. The traditional method ology often includes long incubations (6-24h) and extensive manual steps. An on-chip enzymatic reactor proving rapid protein digestion is presented. Mesoporous carbon nitride was utilized as a supporting material for trypsin. Trypsin was immobilized on mesoporous graphitic carbon nitrides hollow spheres to generate a biocatalyst for protein digestion. Due to the abundant surface amine functionality, high loading of trypin (998 mg/g) on supports was achieved. Immobilized trypsin-embeding stationary phase within microchannel was prepared using in-situ photopolymer as a frit in microchannel., enabling in-situ digestion of proteins such as cytochrome C and myoglobin. The generated peptides were analyzed and identified by mass-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This carbon nitride integrated microfluidic device has the advantages of short digestion time with retention of enzymatic activity, reuse of enzyme and could be combined with other on-chip processes. |
| 저자 |
이은주, 전영시, 홍원희
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| 소속 |
한국과학기술원 |
| 키워드 |
mesoporous carbon nitirde; proteolysis; trypsin; microfluidic
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| E-Mail |
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| 원문파일 |
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