| 초록 |
For successful stem cell therapy, it is important to track the fate of implanted stem cells. The MRI imaging modality using contrast agents such as superparamagnetic iron oxide (SPIO) can be a relatively non-invasive real time tracking method as compared to autopsy or biopsy. However, existing commercialized SPIO showed low uptake efficiency on human mesenchymal stem cells(hMSCs). In this study, we evaluated the uptake of SPIO on hMSCs, and maintenance of their multi-lineage differentiation potential. The SPIO uptake was increased as increasing exposure time of SPIO and uptake efficiency of H-SPIO was higher than that of dextran-SPIO. The uptake of H-SPIO did not affect the viability and proliferation of hMSCs. Most importantly, the differentiation potential of hMSCs into adipogenic and osteogenic lineage was maintained for 14 days after UFH-SPIO uptake. Our results indicate that the H-SPIO can be used as a potential contrast agent for stem cell therapy. |
