| 초록 |
Polylactide (PLA) has been widely studied as a biodegradable polymer in biomedical field. Heparin is known to modulate the biological activity of heparin-binding growth factors and cytokines. The optional binding of the growth factors to heparin results in a fine control of the proteins and cells. In the previous study, we have developed a star-shaped PLA-heparin conjugate which can be applied as blood/cell compatible biodegradable polymer for tissue engineering. Tetronic® is thermosensitive nonionic tetrafunctional block copolymer with four PEO-PPO blocks joined together by a central ethylene diamine bridge, having diverse applications in various biomedical fields ranging from drug delivery and medical imaging to management of vascular disease and disorders. The objective of the present study is to introduce the thermo-responsive property to PLA-heparin conjugate by using thermosensitive multifunctional Tetronic® for pharmaceutical and biomedical applications as an injectable material for cell and drug delivery. Tetronic®-PLA copolymer was synthesized by ring opening polymerization of L-lactide with Tetronic® using stannous octoate. Then, heparin was coupled to Tetronic®-PLA copolymer by N-(dimethylamino) pyridine (DMAP) and dicyclohexylcarbodiimide (DCC). The synthesized Tetronic®-PLA-heparin conjugate was characterized by FT-IR, NMR, GPC and colorimetric assay. The thermo-responsive behaviors and in-vitro assays are now under investigation. |
